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A major factor influencing the fixation process is tissue section size. For both FFPE and FF procedures, the diffusion rate of formalin perfusion or the freezing process limits the maximum tissue ...
For routine paraffin processing and most stains, neutral buffered 10% formalin is a good choice. In general, mouse tissues should be fixed in formalin for 24 to 48 hours, but can be left much longer.
We all know that proper tissue processing is a key element to downstream staining quality, but do you know how to properly assess if your laboratory’s tissue processing is effective? In this webinar, ...
From identifying optimal steps to high-quality tissue processing to understanding the best way to reprocess a specimen, Robin will help you solve your most challenging tissue-processing dilemmas. This ...
Overcrowding can inhibit proper processing, and it isn't possible to get that many tissues positioned in the block for good sectioning anyway. This one should have about 1/2 or 2/3 of the amount of ...
Buffered formalin needs to be used during fixation and processing, and tissue needs to be cut thin for fixation and processing into paraffin.
What are ways to retrieve antigen? The antigen retrieval is based on several variables, such as target antigen, antibody, tissue type, and duration and method of fixation.
The Paragon 28® TenoTac™ Soft Tissue Fixation System was designed to provide surgeons a more simplified and reproducible option for tendon transfers of the lesser toes.
More information: Phung N. Thai et al, Protocol for mouse carotid artery perfusion for in situ brain tissue fixation and parallel unfixed tissue collection, STAR Protocols (2025).
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